|
KMID : 0368419810240010013
|
|
Journal of Plant Biology
1981 Volume.24 No. 1 p.13 ~ p.19
|
|
|
The Effects of Ammonium Citrate and Ammonium Succinate on the Growth of Cells and Nitrogen Absorption in Korean Ginseng Suspension Cultures
|
|
金洪成/Kim, Hong Seong
金明苑/蘇祥燮/姜榮熹/Kim, Myong Won/So, Sang Sup/Kang, Young Hee
|
|
|
Abstract
|
|
|
In order to investigate the effects of ammonium citrate and ammonium succinate on the growth and absorption of nitrogen compounds supplied in the medium, Korean ginseng (Panax ginseng C. A. Meyer) calli were suspension cultured in MS medium with various concentrations of ammonium citrate and ammonium succinate.
When Korean ginseng calli were cultured with 10mM ammonium citrate, 10mM ammonium succinate, and 10mM ammonium nitrate (control) in MS media as the nitrogen sources, the growth, NO_3-N absorption and total nitrogen content of the Korean ginseng cells were greatest in the ammonium citrate and ammonium succinate concnetrations. When Korean ginseng calli were cultured with 5mM ammonium citrate and 5mM ammonium succinate, the growth and nitrogen content were superior to those of the control: however, NO_3-N and NH_4-N absorptions were similar to those of the control. In conclusion, the 10mM ammonium citrate and 10mM ammonium succinate may be better able to facilitate the growth and NO_3-N utilization and uptake than 5mM ammonium citrate and 5mM ammonium succinate concentrations. The concentrations of ammonium citrate and ammonium succinate may be varied in order to obtain the desired effects to facilitate the growth of the ginseng callus.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|